Chloroplast DNA Binding Proteins and Chloroplast Nucleoid
نویسندگان
چکیده
منابع مشابه
DNA gyrase is involved in chloroplast nucleoid partitioning.
DNA gyrase, which catalyzes topological transformation of DNA, plays an essential role in replication and transcription in prokaryotes. Virus-induced gene silencing of NbGyrA or NbGyrB, which putatively encode DNA gyrase subunits A and B, respectively, resulted in leaf yellowing phenotypes in Nicotiana benthamiana. NbGyrA and NbGyrB complemented the gyrA and gyrB temperature-sensitive mutations...
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Chloroplast gene expression is mainly regulated at the post-transcriptional level by numerous nuclear-encoded RNA-binding protein factors. In the present study, we focus on two RNA-binding proteins: cpRNP (chloroplast ribonucleoprotein) and PPR (pentatricopeptide repeat) protein. These are suggested to be major contributors to chloroplast RNA metabolism. Tobacco cpRNPs are composed of five diff...
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The 3-dimensional structure of the plastid nucleoid was determined from serial sections of the plastid of dark-grown, greening, and light-grown cells of Ochromonas danica. In lightgrown and greening cells, the chloroplast nucleoid forms a continuous cord or ring which closely follows the rim of each lateral lobe of the chloroplast and is continuous across the top and bottom of the bridge connec...
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After solubilization of stroma-freed chloroplasts with deoxycholate, the lipids and the detergent used are separated from the proteins by gel filtration. In this way not denatured pigment-containing protein preparations were obtained. The particles in fraction 1 exhibited a molecular weight of 600 000 and contained an average of 25 chlorophyll molecules. The circular dichroism spectrum showed e...
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Cultured leaf disks from young spinach leaves have been used to study chloroplast DNA synthesis in relation to chloroplast growth and repli cation. Autoradiographs prepared from thin sections show th a t labelling throughout disks is uniform. We consider th a t cultured leaf disks serve as a model system for studies of cell expansion and chloroplast replication during the development of spinac...
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ژورنال
عنوان ژورنال: Nippon Nōgeikagaku Kaishi
سال: 1997
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.71.1173